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Solange Oliveira
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Present position
Academic degrees
Current Teaching
Current Research
Previous positions
Some links
Address
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Present
position
- Assistant professor
Department of Biology ·
- Researcher
Soil Microbiology Laboratory - Instituto de Ciências Agrárias e Ambientais Mediterrânicas (ICAAM) University of Évora
Academic
degrees
- Ph.D. in Biochemistry (Genetics, Virology
and Immunology), Faculty of Science, University of
- Degree in Biochemistry, Faculty of
Science-University of
- Genetics
Graduations in Biology and Biochemistry
- Molecular Biology
Graduation in Biology and Biochemistry
- Applied Molecular Biology
European Master of Science in Nematology (EUMAINE)
Current
Research
Research interests
·
Molecular
biology of plant viruses
·
Molecular
biology of nematodes and associated bacteria
·
Molecular
phylogeny
Experimental Techniques
·
Microbiology
·
Bioinformatics
Projects in Rhizobia -
http://www.rhizobia.uevora.pt/index.html
·
Rhizobia nodulating chickpea:
genetic characterisation and relation to strain origin and symbiotic efficiency
(PRAXIS/P/AGR/11129/1998) (7/1999-7/2001), PI - Mário
de Carvalho, University of Évora
·
Diversity of rhizobia natural
populations in Portugal: strains tolerant to environmental stress conditions”
(POCTI/BME/44140/2002) (10/ 2003-10/2006) PI - Solange
Oliveira
·
Improvement of Rhizobia by
Genetic Modification with Stress Response Genes (PTDC/BIO/80932/2006), co-financed by Fundação para a Ciência e a Tecnologia and FEDER Programa
POCI 2010. PI - Solange Oliveira. Ongoing since
January 2009.
Participation
in other Projects
·
Expression of metalloproteins
from Desulfovibrio gigas
(PRAXIS/P/BIA/11074/1998). PI - C. Rodrigues-Pousada,
ITQB-UNL (6/1999-6/2001).
·
Design and Optimization for virus diagnosis in Olea Europaea L.
for plant sanitary certification (PCTI/1999/AGR/36230). PI -
M. I. Clara,
University of Évora (1/ 2001-1/2004).
·
Genes involved in bioenergetic
mechanisms in the sulphate reducing bacterium Desulfovibrio gigas (POCTI/BME/37480/2001). PI - C. Rodrigues-Pousada, ITQB, UNL (9/ 2003-9/2006).
·
Programa de Acção
Nacional para Controlo do Nemátode da Madeira do Pinheiro: "A doença do
nemátode da madeira do pinheiro “Pine Wilt Disease”, PWD, Bursaphelenchus xylophilus" financed by Instituto de
Financiamento da Agricultura e Pescas (IFAP) and Autoridade Florestal Nacional
(AFN) Linha 3 (2008-ongoing).
·
The role of weeds mycorrhiza
on the growth of winter crops and interactions with native rhizobia under
Mediterranean conditions (PTDC/AGR-PRO/111896/2009). PI – M. Carvalho, University of Évora (1/2/2011 – 31/1/2014).
·
FP7- People -2009-IRSES “Biocontrol
and Bioremediation agents and their role in Agriculture and Forest health”
(1/03/2011-ongoing)
Other Collaborations
·
J. Peter W. Young,
·
Encarna Vélazquez, Universidad
de Salamanca, Spain - Molecular phylogeny,
rhizobia
·
Raul Rivas, Universidad de Salamanca, Spain - Molecular phylogeny, rhizobia
·
Isabel Duarte Maçãs,
Estação Nacional de Melhoramento de Plantas (ENMP), Portugal - Chickpea
·
Manuel Mota,
·
Pedro Mateos, Universidad
de Salamanca, Spain – Rhizobia
·
Bernard R. Glick, University of Waterloo, Canada -
Plant Growth Promoting Bacteria, acdS gene
·
Rogério Tenreiro,
Universidade de Lisboa, Faculdade de Ciências, Portugal - Microbial Taxonomy
Ph.D. Students supervision
Graduated
-Manuela Broco “Análise e
expressão genética de proteínas de oxidação-redução de Desulfovibrio
gigas” ITQB-UNL, FCT (2005)
(co-supervisor)
-Rute Rodrigues “Análise
e expressão de genes envolvidos em mecanismos bioenergéticos de Desulfovibrio
gigas” ITQB-UNL, FCT (2006)
(co-supervisor)
-Marta Laranjo “Genetic
Diversity and Symbiotic effectiveness of Chickpea rhizobia
strains” Universidade de Évora/ICAM,
FCT (2007) (supervisor)
-Rosário Félix
“Caracterização biomolecular de vírus de Olea europaea L. e concepção de
métodos de diagnóstico viral aplicados à cultura” Universidade de Évora/ICAM
(2008) (co-supervisor)
-Joana Cardoso “Molecular
characterization of necroviruses isolated Olea europeae L.” Universidade de
Évora/ICAAM, FCT (2010) (supervisor)
-Ana Alexandre
“Temperature stress tolerance in chickpea rhizobia” Universidade de
Évora/ICAAM, FCT (2010) (supervisor)
In
Course
-Clarisse
Brígido “Avaliação da tolerância ao stress salino de estirpes de rizóbio de
grão-de-bico” Universidade de Évora/ICAM, FCT (2007- ) (supervisor)
-Vera
Valadas “Caracterização morfológica e taxonomia molecular de nemátodes
entomopatogénicos (Nematoda: Steinernematidae e Heterothabditidae) em Portugal
Continental” Universidade de Évora/ICAM, FCT (2006- ) (co-supervisor)
-Carla Varanda
“Caracterização molecular da transmissão de necrovirus (Tombusviridae) de Olea europaea L. por fungos vectores”
Universidade de Évora/ICAM, FCT (2007- )
(co-supervisor)
Master
Students supervision
In Course
-Francisco Nascimento “ACC deaminase in Mesorhizobium: prevalence and effect on chickpea-rhizobia symbiosis” Universidade
de Évora/ICAAM (co-supervisor)
Publications
1-Oliveira,
S. and Costa, J. V. (1995) "Replication of transfected
plasmid DNA by cells infected with african
swine fever virus" Virology, 207, 392-399.
2-Gomes,
C., Silva, G., Oliveira, S., LeGall, J., Liu, M-Y.,
Xavier, A., Rodrigues-Pousada, C. e Teixeira, M. (1997) “Studies on the redox
centers of the terminal oxidase
from Desulfovibrio gigas
and evidence for its interaction with rubredoxin” The
Journal of Biological Chemistry, 272, 22502-22508.
3-Silva,
G., Oliveira, S., Gomes, C., Pacheco,
4-Broco,
M., Oliveira, S., Silva, G., Agostinho, M., Rodrigues-Pousada, C. (2000) “A DNA
fragment of Desulfovibrio gigas genome containing replication origin related
genes” DNA Sequence, 11(1-2), 119-124.
5-Agostinho,
M., Oliveira, S., Broco, M., Liu, M.-Y., LeGall, J., Rodrigues-Pousada, C.
(2000) “Molecular cloning of the gene encoding flavoredoxin, a flavoprotein
from Desulfovibrio gigas” Biochemistry and Biophysical Research Communications,
272 (3), 653-656.
6-Frazão,
C., Silva, G., Gomes, C.M., Matias, P., Coelho, R., Sieker, L., Macedo, S.,
Liu, M.Y., Oliveira, S., Teixeira, M., Xavier, A.V., Rodrigues-Pousada, C.,
Carrondo, M.A., LeGall, J. (2000) “Dioxygen reduction in strict anaerobes. Structure of Desulfovibrio gigas rubredoxin:oxygen oxidoreductase” Nature Structural Biology, 7 (11),
1041-1045.
7-Silva,
G., Oliveira, S., LeGall, J., Xavier, A.V., Rodrigues-Pousada, C. (2001) “Analysis of the Desulfovibrio gigas
Transcriptional Unit Containing Rubredoxin (rd) and Rubredoxin-Oxygen Oxidoreductase
(roo) Genes and Upstream ORFs” Biochemical and
Biophysical Research Communications, 280 (2):491-502.
8-Laranjo,
M., Rodrigues, R., Alho,
L., and Oliveira, S. (2001)"Rhizobia of chickpea from Southern Portugal:
symbiotic efficiency and genetic diversity" Journal of Applied
Microbiology, 90:4, 662-667.
9-Laranjo,
M., Branco, C., Soares,
R., Alho, L., Carvalho, M. and Oliveira, S. (2002) “Comparison of
chickpea rhizobia isolates from diverse Portuguese natural populations based on
symbiotic effectiveness and DNA fingerprint”
Journal of Applied Microbiology 92:6, 1043-50.
10-Victor,
B., Vicente, J., Rodrigues, R., Oliveira, S., Rodrigues-Pousada, C., Frazão,
C., Gomes, C., Teixeira, M., Soares,
C.(2003) “Docking and electron transfer studies
between rubredoxin and rubredoxin:oxygen
oxidoreductase” Journal of Biological Inorganic
Chemistry, 8 (4): 475-488.
11-Rodrigues,
R., Valente, F., Pereira, I. Oliveira, S., Rodrigues-Pousada,C. (2003) “A
novel membrane-bound Ech [NiFe]
hydrogenase in Desulfovibrio
gigas” Biochemical and Biophysical Research
Communications, 306
(2), 366-75.
12-Laranjo,
M., Machado, J., Young, J.P.W., Oliveira, S. (2004) “High diversity of chickpea
Mesorhizobium species isolated in a Portuguese
agricultural region” FEMS Microbiology Ecology, 48 (1), 101-107.
13-Cardoso,
J.M.S., Félix, M.R., Oliveira, S., Clara, M.I.E.
(2004) “A Tobacco necrosis virus D isolate from Olea europaea L.: viral characterization and coat protein
sequence” Archives of Virology, 149, 6, 1129-1138.
14-Cardoso, J.M.S.,
Félix, M.R., Clara, M.I.E,. Oliveira, S. (2005) "The complete genome sequence
of a new necrovirus isolated from Olea
europaea L" Archives of Virology, 150, (4):
815–823.
15-Félix,
M.R., Cardoso, J.M.S., Oliveira, S., Clara, M.I.E. (2005) "Viral
properties, primary structure and phylogenetic
analysis of the coat protein of Olive latent virus 1 isolate from Olea europaea L." Virus
Research 108: 195-198.
16-Broco,
M., Rousset, M., Oliveira, S. and Rodrigues-Pousada, C. (2005)
“Deletion of flavoredoxin gene in Desulfovibrio
gigas reveals its participation in thiosulfate reduction”
FEBS Letters, 579, 4803-4807.
17-Broco,
M., Marques, A., Oliveira, S., Rodrigues-Pousada, C. (2005) “Characterisation of the 11 kb DNA
region adjacent to the gene encoding Desulfovibrio gigas flavoredoxin” DNA Sequence,
16(3): 207-216.
18-Félix, M.R. Cardoso,
J.M.S.. Varanda, C.M.R Oliveira S. and Clara, M.I.E. (2005)“Complete nucleotide sequence of an Olive latent
virus 1 isolate from olive trees” Archives of Virology, 150 (11): 2403-2406.
19-Rodrigues,
C., Laranjo, M. and Oliveira, S. (2006) "Effect
of heat and pH stress in the growth of chickpea mesorhizobia"
Current Microbiology, 53, 1–7.
20-Alexandre, A., Laranjo,
M. and Oliveira, S. (2006) "Natural populations of chickpea rhizobia
evaluated by antibiotic resistance profiles and molecular methods"
Microbial Ecology, 51, 128-136.
21-Félix,
R., Rodrigues, R., Machado, P., Oliveira, S., Rodrigues-Pousada, C. (2006) “A chemotaxis operon in the bacterium Desulfovibrio
gigas is induced under several growth conditions” DNA
Sequence, 17(1), 56-64.
22-Machado,
P., Félix, R., Rodrigues,
R., Oliveira, S., Rodrigues-Pousada,
C. (2006)“Characterisation and expression analysis of
the cytochrome bd oxidase operon from Desulfovibrio gigas”, Current
Microbiology, 52, 274-281.
23-Rodrigues,
R., Vicente, J., Félix, R. Oliveira, S., Teixeira, M., Rodrigues-Pousada, C., (2006) ”Desulfovibrio gigas Flavodiiron protein affords in vivo protection against nitrosative stress” Journal of Bacteriology, 188 (8), 2745–2751.
24-Brígido, C.,
Alexandre, A., Laranjo, M., Oliveira, S. (2007)"Moderately acidophilic
Mesorhizobia isolated from chickpea (Cicer arietinum L.)" Letters in Applied Microbiology 44 (2):
168-174.
25-Rivas,
R., Laranjo, M., Mateos,
P.F., Oliveira, S., Martınez-Molina E., and
Velazquez, E. (2007)“Strains of Mesorhizobium amorphae and Mesorhizobium tianshanense, carrying symbiotic genes
of common chickpea endosymbiotic species, constitute
a novel biovar (ciceri) capable of nodulating Cicer arietinum” Letters in Applied Microbiology, 44 (4):
412-418.
26-Broco, M., Soares,
C.M., Oliveira, S., Mayhew, S.G., Rodrigues-Pousada, C. (2007) “Molecular
determinants for FMN-binding in Desulfovibrio gigas flavoredoxin” FEBS Letters
581, 4397–4402.
27-Félix, M.R., Cardoso,
J.M.S., Oliveira, S., Clara, M.I.E. (2007) “Biological and Molecular
Characterization of Olive latent virus 1” Plant Viruses 1(2): 170-177.
28-Laranjo,
M., Alexandre, A., Rivas, R., Velázquez, E., Young,
J. P. W. and Oliveira, S. (2008) "Chickpea rhizobia symbiosis genes are
highly conserved across multiple Mesorhizobium species" FEMS Microbiology Ecology,66 (2): 391-400.
29-Alexandre,
A., Laranjo, M., Young, J. P. W. and Oliveira, S.
(2008) "dnaJ
is a useful phylogenetic marker for alphaproteobacteria" International Journal of
Systematic and Evolutionary Microbiology,58 (12):
2839-2849.
30-Alexandre,
A., Brígido, C., Laranjo,
M., Rodrigues, S. and Oliveira, S. (2009)
"Survey of chickpea rhizobia diversity in Portugal reveals the
predominance of species distinct from Mesorhizobium ciceri and Mesorhizobium mediterraneum",Microbial Ecology,58, 930-941.
31-Cardoso,
J.S., Félix, M.R., Clara, M.I., Oliveira, S. (2009)
“Complete genome sequence of a Tobacco necrosis virus D isolate from olive
trees”, Archives of Virology, 154 (7): 1169-1172.
32-Valadas, V., Vieira,
P., Oliveira, S., Mota, M. (2009) “First report of the genus Heterorhabditis (Nematoda: Heterorhabditidae)
from continental Portugal”, Helminthologia, 46(1): 45-48.
33-Varanda,
C., Cardoso, J.M.S., Félix, M.R., Clara, M.I. (2010)
“Multiplex RT-PCR for detection and identification of three necroviruses
that infect olive trees”, European Journal of Plant Pathology, 127(2): 161-164.
34-Alexandre, A. and Oliveira, S. (2011) "Most
heat-tolerant rhizobia show high induction of major chaperone genes upon
stress", FEMS Microbiology Ecology, 75, 28-36.
35-Laranjo, M. and Oliveira, S. (2011) "Tolerance
of Mesorhizobium
type strains to different environmental stresses", Antonie
van Leeuwenhoek, 99, 651-662.
Book Chapters
-Laranjo, M. and Oliveira,
S. (2006) "Rhizobial Strain Improvement: Genetic
Analysis and Modification" In "Microbial Biotechnology in Agriculture
and Aquaculture" Vol II, pp. 225-260. (Editor R.
C. Ray). Science Publisher Inc,
-Oliveira, S., Laranjo,
M., Alexandre, A. (2008). "Molecular Tools in Microbial
Ecology" In "Ecology Research Trends", chapter 4, pp. 161-183. Edited by Laia Diaz and Marta Perez, Nova Science Publishers, Inc.,
New York, USA.
Departmental responsibilities
-Head of Department of Biology, 2003/2005
-Head of Biology Graduation Commission, 2000/2003
-Head of the Commission of PhD Programme
in Biology, 2009/2010
Previous positions
- Post-Doc – ITQB
- Project assistant, Virology Laboratory -Gulbenkian Institute of Science (IGC)
- Ph.D. fellowship from Junta Nacional de Investigação Científica e Tecnológica (JNICT)
- Ph.D. fellowship from Calouste
Gulbenkian Foundation (FCG)
Advanced PubMed
Search http://www.ncbi.nlm.nih.gov/PubMed/medline.html
EMBO http://www.embo.org/
TIGR Microbial Database http://www.tigr.org/tdb/mdb/mdbcomplete.html
Bioinformatic tools:
http://www.medkem.gu.se/edu/translat.html
Isabel Solange Martins de Oliveira
Departamento de Biologia · Universidade de Évora
Núcleo da Mitra · Apartado 94 · 7002-554 Évora · PORTUGAL
Phone +351-266 760 881 · Fax +351-266 711 231